Ultrasonic Surface Treatment
Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilize proteins, glycoproteins, or nucleic acids. However, the use of nitrocellulose coating for microarray analysis, such as found in microfluidic systems, is limited by the physical properties of nitrocellulose and the complicated manufacturing process used to make nitrocellulose-based assays. Our ultrasonic surface treatment is the invention for forming a nitrocellulose film on a carrier. The solvent used to particalize the nitrocellulose solution that is evenly sprayed on a wide variety of carrier, such as silicon rubber, glass, organic or inorganic polymer or paper by adjustment of the air pressure. The process for forming a nitrocellulose film on a carrier in accordance with the present invention reduces the material loss and small impact on the integrity of the biospecimen relative to conventional processes in which nitrocellulose paper is cut to desired sizes corresponding to the shape of the carrier.